For your reference we have cataloged below a number of published articles about the features, benefits, and uses of Hemo-De. We invite you to read them and learn how Hemo-De can help your laboratory.
Hemo-De Usage Article #1
Title Use of Hemo-De to eliminate toxic agents
used for concentration and trichrome staining of intestinal parasites
Authors W.E. Aldeen and D. Hale
Associated Regional and University Pathologists, Inc.,
Salt Lake City, Utah 84108.
Publication Journal of Clinical Microbiology, Jul 1992, 1893-1895, Vol 30, No. 7
Copyright © 1992 by the American Society for Microbiology. All rights reserved.
Abstract In a blind comparison, 465 randomly collected clinical fecal specimens were examined. Hemo-De was found to be an excellent replacement for ethyl acetate in the concentration procedure and for carbol-xylene and xylene in the trichrome staining procedure. Elimination of toxic reagents, combined with its lower cost, makes Hemo-De the preferred choice in routine parasitology examinations.
Download Full Article   pdf

Hemo-De Usage Article #2
Title Gutta-Percha Softening: "Hemo-De" as a Xylene Substitute.
Authors Metzger, Zvi DMD; Marian-Kfir, Vered DMD; Tamse, Aviad DMD
Journal of Endodontics. 26(7):385-388, July 2000.
Publication Copyright © 2005, American Association of Endodontists. All rights reserved.
Published by Lippincott Williams & Wilkins.
Abstract The putative hazardous nature of chloroform and xylene implies that safer substitutes should be considered. Standard cylindrical gutta-percha samples were immersed in Hemo-De, a xylene substitute, for 60 s at 37[degrees]C. Weight loss of a sample, after drying, was used as a measure of its solubility. Master and accessory cone gutta-percha of three brands were compared. The highest solubility of all kinds of gutta-percha was in chloroform, which served as a positive control. The average solubility of all samples in xylene and Hemo-De was 61% and 52% of that in chloroform, respectively. DMS gutta-percha was more soluble than of Hygienic and DeTrey. Master cone gutta-percha of all brands was more soluble than that of their accessory cones. These results indicate that (a) large differences exist in the solubility of gutta-percha and (b) Hemo-De dissolved gutta-percha in a range similar to that of xylene and may be considered as a potential substitute for this organic solvent.
Download Full Article  pdf

Hemo-De Usage Article #3
Title Use of a Single Slide Trichrome-Stained Concentrate
for the Detection of intestinal Parasites
Stained Concentration Procedure for Ova and Parasites
Authors Devon C. Hale, MD, Karen Carroll, MD,
James R Kucera, MS, and William E. Aldeen, MBA

American Journal of Clinical Pathology, Vol. 106, No. 2, August 1996.
Publication Copyright © 1996, American Society of Clinical Pathologits. All rights reserved.
Published by Lippincott-Raven Publishers.
Abstract The detection and identification of intestinal parasites was studied using a single slide of a trichrome-stained concentrate made from a cupircpolyvinal alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately stain the concentrate with a modified-trichrome stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified trichrome-stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated trichrome stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
Download Full Article  pdf

Hemo-De Usage Article #4
Title Biodegradable, effective substitute for xylene in the Ehrlich indole procedure
Authors JM Miller, MD Miller, PE Driscoll and P Miller
Diagnostic Microbiology Section, Centers for Disease Control and Prevention, Atlanta, GA 30333.
Publication Journal of Clinical Microbiology, Aug 1994, 2028-2030, Vol 32, No. 8
Copyright © 1994 by the American Society for Microbiology. All rights reserved.
Abstract Three extracting reagents were compared for effectiveness in the Ehrlich indole procedure: xylene (the recommended reagent), Hemo-De (a terpene-based product containing d-limonene), and Micro-Clear (an isoparaffinic hydrocarbon). Thirty-three strains representing 12 species of indole-positive aerobes or facultative anaerobes and 50 strains representing 11 species of indole-positive strict anaerobes were tested using the three reagents. Xylene extraction allowed indole detection in all of the isolates tested. Micro-Clear allowed detection in all of the aerobic isolates and in 49 of 50 anaerobes. Hemo-De allowed indole detection in 32 of 33 aerobes and in 49 of 50 anaerobes. There was no significant difference in the results among the reagents. Because Micro-Clear is biodegradable, nonflammable, noncarcinogenic, and odorless, we feel that this product should be considered a safe and effective substitute for xylene in the Ehrlich indole procedure.
Hemo-De Material Safety Data Sheet

home | products | purchase | distributors | articles | contact us

Copyright © Scientific Safety Solvents
Keller, Texas 76248